Synthesis of a Tethered myo -Inositol (1,3,4,5,6)Pentakisphosphate (IP5) Derivative as a Probe for Biological Studies

M Gregory; B Catimel; MX Yin; M Condron; AW Burgess; AB Holmes

Journal article

Synthesis of a Tethered myo -Inositol (1,3,4,5,6)Pentakisphosphate (IP5) Derivative as a Probe for Biological Studies

M Gregory, B Catimel, MX Yin, M Condron, AW Burgess, AB Holmes

Synlett | GEORG THIEME VERLAG KG | Published : 2016

DOI: 10.1055/s-0035-1560381

Abstract

There is sufficient evidence to suggest that myo-inositol pentakisphosphate is a vital intermediate species in higher inositol phosphate metabolism, however, its biological roles and physiological function in cells remain uncertain. A tethered myo-inositol pentakisphosphate (IP5) derivative with a terminal amine group is synthesised allowing facilitated immobilisation onto M-270 magnetic Dynabeads for pull-down experiments and biosensor chip preparation for surface plasmon resonance studies. The probes are validated by both pull-down and surface plasmon resonance (SPR) studies of the known binding protein GRP-1 (general receptor for phosphoinositides 1), and furthermore by SPR studies of pro..

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University of Melbourne Researchers

Antony Burgess Author

Andrew Holmes Author

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Grants

Awarded by Australian Research Council

Awarded by NHMRC Program

Funding Acknowledgements

This work was supported by the Australian Research Council, Discovery Project DP1094497 and the University of Melbourne (MIRS and MIFRS scholarships to M.G.). We thank Dr. N. Aberle (University of Melbourne) for his interest in this work. An NHMRC Program grant 487922 provided access to the facilities in the Walter and Eliza Hall Institute.